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Preparing Curcumin Nanoparticles And Examine Its Activity With Combine Magnetic Field Again Tumors And Microbes In Vitro

Preparing Curcumin Nanoparticles And Examine Its Activity With Combine Magnetic Field Again Tumors And Microbes In Vitro

ABSTRACT

Cancer still considered one of the major health problems and causes of death globally. Prostate cancer (PC) is highly prevalent among malignancies in men in the Western world and Prostate cancer (PC) is the third cause of death from in men. Efficient treatment for PC includes surgery, radiation, and chemotherapy. Most of cancer treatment regimens focus primarily on chemotherapy as the treatment of choice for advanced or metastatic disease. Curcumin (diferuloylmethane) is a polyphenol derived from the plant Curcuma longa, commonly called turmeric. Extensive research over the last 50 years has demonstrated this Polyphenols play an important role in the maintenance of health and prevention of diseases. Numerous reports suggest that this natural compound possesses therapeutic benefit such as In this study to enhance the solubility and bioavailability of curcumin by fabricating its nanoparticles, and examine in vitro the curcumin and nanocurcumin against prostate cancer cell line (PC3), human embryonic kidney cell line (HEK). On the other hand, we examined the curcumin with combined magnetic field (CUR-MF) and nanocurcumin with combined magnetic fieIn this study also the fabricating nanoparticles of curcumin (nanocurcumin) and curcumin examine against human erythrocytes (hemolysis %). Moreover, the effect of magnetic field with combined curcumin (MF-CUR) and nanocurcumin (NANOCUR-MF) on human erythrocytes it was also investigated.

In this study also, we examined the curcumin and nanocurcumin with and without combined magnetic field as antimicrobial agent, by evaluate the minimum bacteria concentration (MBC), and the results indicate that curcumin and nanocurcumin inhibited the bacteria growth with differentiation toxicity for nanocurcumin.

 

In conclusion, both curcumin and nanocurcumin displayed significant selectivity against cancer cells when compared with their activity against normal mammalian cells. Moreover, curcumin and nanocurcumin demonstrate activity as antimicrobial agent with very low hemolysis %. Therefore could prove to be potential candidate for anticancer and or antimicrobial drug development in future studies. On the other hand, the CUR-MF and NANOCUR-MF against both of cancer and normal cell lines, give indication effect to reduce the cell viability comparing with control, with more efficiency on PC3 than HEK. Moreover, both of CUR-MF and NANOCUR-MF make to increase the hemolysis % for RBCs after exposed overnight with slightly increasing after 60 min exposure. Furthermore, both of CUR-MF and NANOCUR-MF demonstrate reducing of CFU (colony forming units) after exposed overnight comparing with control. Although the all previous finding, but the effect MF against cell lines, human erythrocytes, and antimicrobial is in need more studies indeed.

 

 

 

Menyediakan Nanopartikel Curcumin Dan Memeriksa Aktiviti Dengan Menggabungkan Medan Magnet Lagi Tumor Dan Mikrob Dalam Vitro

 

ABSTRAK

 

Secara global, kanser atau barah masih dianggap satu daripada masalah kesihatan utama dan menyebabkan kematian. Kanser prostat (prostate cancer, PC) adalah amat prevelans dalam kalangan kaum lelaki di Barat. Di samping itu, ia juga merupakan penyebab ketiga kematian dalam kalangan kaum lelaki. Rawatan yang berkesan bagi PC termasuklah pembedahan/surgeri, pancaran/radiasi, dan kemoterapi. Kebanyakan regimen rawatan kanser memberi tumpuan terutamanya pada kemoterapi sebagai rawatan pilihan bagi penyakit metastatik. Curcumin (diferuloilmetana) merupakan polifenol yang diterbitkan daripada pokok Curcuma longa, yang dikenali juga sebagai kunyit. Penyelidikan yang dijalankan sejak 50 tahun lalu menunjukkan bahawa polifenol memainkan peranan penting dalam pengekalan kesihatan dan pencegahan penyakit. Pelbagai laporan yang ada mencadangkan bahawa sebatian asli ini menunjukkan manfaat terapeutik seperti antitumor, anti-inflamasi / anti-radang dan aktiviti anti-oksidan.

 

Kajian ini bertujuan meningkatkan kebolehlarutan dan kebolehbiosediaan curcumin melalui fabrikasi nanopartikelnya dan mengkaji in vitro daripada curcumin dan nanocurcumin terhadap lini sel kanser prostat (PC3), dan lini sel ginjal embrio manusia. Dalam kata lain, kami mengkaji curcumin yang digabung dengan medan magnet (CUR-MF) dan nanocurcumin yang digabung dengan medan magnet (NANOCUR-MF) terhadap PC3 dan HEK selepas didedahkan selama 6 jam terhadap medan magnet (MF) 8 mT AC

 

Kajian ini juga mengkaji fabrikasi, nanocurcumin dan curcumin terhadap eritrosit manusia (peratus(%) hemolisis). Di samping itu, kesan medan magnet dengan gabungan curcumin (MF-CUR) dan nanocurcumin (NANOCUR-MF) terhadap eritrosit manusia juga dikaji..Kajian ini juga turut mengkaji curcumin dan nanocurcumin dengan / dan tanpa digabung medan magnet sebagai agen antimikrob, melalui penilaian kepekatan bakteria minimum ( minimum bacteria concentration, MBC), dan keputusan menunjukkan bahawa curcumin dan nanocurcumin mampu merencat pertumbuhan bakteria dengan ketoksikan nanocurcumin yang berbeza.

 

Sebagai kesimpulan, kedua-dua curcumin dan nanocurcumin memapatkan kebolehpilihan yang signifikan terhadap sel kanser apabila dibandingkan dengan aktiviti mereka terhadap sel mamalia normal. Di samping itu, curcumin dan nanocurcumin menunjukkan aktiviti sebagai agen antimikrob dengan peratus hemolisis yang amat rendah. Justeru, dapat dibuktikan bahawa ia berpotensi sebagai antikanser dan / atau perkembangan drug antimicrob dalam kajian selanjutnya pada masa depan Dalam kata lain, CUR-MF dan NANOCUR-MF terhadap kedua-dua lini sel normal dan lini sel kanser, memberikan indikasi kesan untuk mengurangkan perbandingan viabiliti sel dengan kawalan, dengan lebih berkesan terhadap PC3 daripada HEK. Tambahan pula, kedua-dua CUR-MF dan NANOCUR-MF mampu meningkatkan peratus hemolisis RBCs selepas didedahkan semalaman dengan kenaikan yang sedikit selepas 60 minit pendedahan. Selanjutnya, kedua-dua CUR-MF dan NANOCUR-MF menunjukkan pengurangan CFU (colony forming units) selepas didedahkan semalaman dibandingkan dengan kawalan. Sesungguhnya, di samping dapatan di atas, kesan MF terhadap lini sel, eritrosit manusia, dan antimicrob masih memerlukan kajian lanjut